Abstract
The analysis of phenols using high-performance liquid chromatography and post-column reaction detection has been studied. The system design utilizes the reaction of ten of the phenols on the U.S. Environmental Priority Pollutant List with 4-aminoantipyrine and potassium ferricyanide. The chromophores that are produced can be quantified at either 509 nm or 470 nm depending upon the structure of the eluting phenol. The separation procedures uses a linear gradient consisting of 0.2% phosphoric acid and methanol. The phosphoric acid concentration is varied from 90% to 20% in 20 min. providing for resolution of each of the ten phenols. The concentration of each of the reagents and the pH used for the post-column reaction have been studied in order to optimize the post-column quantification. In addition two methods for concentrating the phenols in water and waste-water have been examined. The recovery and repeatability of these are presented. A series of influent and effluent samples from a wastewater treatment plant have been assayed using the method presented. The results of this study are discussed.
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