Determination of phenolic and triterpenic compounds in Jatropha gossypiifolia L by Ultra-high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS)

Rudinei Da Silva Silveira,Mariana Tatsch Da Silva Souza,Géssica Domingos Da Silveira,Gabriela Camera Leal,Henrique Faccin,Carine Viana,Thais Ramos Dal Molin,Luciana Assis Gobo,Leandro Machado De Carvalho,Osmar Alves Lameira

doi:10.1590/s2175-97902019000417262

Rudinei Da Silva Silveira, Mariana Tatsch Da Silva Souza + Show 8 more

Open Access

https://doi.org/10.1590/s2175-97902019000417262

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Abstract

This work describes the chemical characterization of extracts of Jatropha gossypiifolia (from Amazonia region) concerning the presence of phenolic and triterpenic compounds using Ultra High Perfomance Liquid chromatography coupled to Mass Spectrometry in Tandem (UHPLC-MS/MS) methods. The studied compounds belong to the most frequently found classes in medicinal plants (triterpenes, flavonoids, flavones, hydroxycinnamic acids, coumarins, catechins and stilbenes), which have been analyzed as chemical and bioactive markers in hydroethanolic and aqueous extracts. Several polyphenolic compounds identified herein are unprecedented in the scientific literature for this species. The chemical markers identified and quantified in the studied extracts of J. gossypiifolia were gallic acid, chlorogenic acid, catechin, caffeic acid, vanillic acid, p-coumaric acid, ferullic acid, rutin, quercitrin, 3-acetylcoumarin, trans-cinnamic acid, quercetin, luteolin, apigenin, kaempferol, chrysin. α-amyrin, β-amyrin and lupeol. As the majority compounds, (+)-catechin, p-coumaric acid, ferulic acid, luteolin, α-amyrin and β-amyrin were found to be present at mg kg-1 levels. J. gossypiifolia extracts presented a high in vitro activity against different reactive oxygen species (hydroxyl, peroxyl, and superoxide anion radicals). Several polyphenolic compound data presented herein are unprecedented in the scientific literature for this plant species. As a result, this plant can be a new source of bioactive molecules for therapeutic purposes.

Highlights

Traditional medicine (TM) is an important and often underestimated part of health services
Medicinal plants represent the greatest sources of therapeutic agents due to the large structural diversity of metabolites produced (Fabricant, Farnsworth, 2001)
J. gossypiifolia presents an important potential for the generation of pharmacological and/or biotechnological products, based on popular uses and biological studies

Summary

Introduction

Traditional medicine (TM) is an important and often underestimated part of health services. In some countries, including Brazil, traditional medicine or non-conventional medicine may be termed complementary medicine. TM has a long history of use in health maintenance and in disease prevention and treatment, for chronic diseases. According to the World Health Organization (WHO) Traditional Medicine Strategy 2014-2023, policies and regulations to promote the safe and effective use of Traditional and complementary medicine (T&CM) products have increased all over the world (WHO, 2016). Herbs are used throughout the world and their use is on the rise, with available data suggesting that the T&CM product market is substantial. The diversity of regulatory categories for T&CM products makes it difficult to assess the size of the market with any degree of accuracy. The output of Chinese materia medica was estimated to be about US$83.1 billion in 2012, annual expenditure on TM in the Republic of Korea was US$7.4 billion in 2009 and out-of-pocket spending for natural products in the United States reached US$14.8 billion in 2008 (WHO, 2016)

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