Abstract
Intravacuolar pH values of phagocytosing mussel blood cells were determined by both in vitro and in vivo tests. Yeast cells stained with pH-sensitive dyes were used to challenge Mytilus hemocytes prepared as cell monolayer on slides or mussels were injected with the yeast suspension. After ingestion by hemocytes, yeast stained with dyes changing their color at pH 5.0 or less turned to the acid hue, whereas those which change at pH 4.0 or less did not react. Consequently, a pH of 4.5 ± 0.5 occurs inside the digestive vacuoles of Mytilus hemocytes.
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