Abstract
A competitive enzyme immunoassay using specific pentachlorophenol (PCP) antiserum covalently coupled to a magnetic particle solid phase has been developed for the detection of PCP in water and soil. The immunoassay allows the quantification of PCP from 100 ppt(parts per trillion, ng/L) in water and 100 ppb (parts per billion, mg/L) in soil. Spike recovery from water samples with no sample pretreatment and from various soil types following a simple extraction technique averaged 105% and 94%, respectively. The method compares favorably with GC/MS and HPLC measurements in water (r=0.980) and soil (r=0.996) samples.
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