Abstract
A bovine corneal endothelial cell plasma membrane vesicle preparation was used to investigate passive Na + transport across the plasma membrane of these cells. Sodium accumulation rate into the vesicle was not dependent on the presence of HCO 3 − or a HCO 3 − gradient, but was stimulated by a trans-vesicle pH gradient. Amiloride, furosemide and DIDS all reduced the rate of Na + accumulation. The data indicate the presence of at least two independent pathways for passive sodium movement across the vesicle: the first probably via a Na +/H + exchanger and the second a furosemide inhibitable Na + entry mechanism. No evidence was found for direct Na +-HCO 3 − coupled transport.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
