Abstract
Abstract Parathion, methyl parathion, EPN, and their corresponding oxons are extracted from plant material with acetonitrile, cleaned up by elution from polyethylene-coated alumina with a 40% acetonitrile solution, partitioned between chloroform and water, and eluted from magnesol with chloroform. Total content is estimated by a spectrophotometric measurement at 400 mμ of the p-nitrophenol produced by alkaline hydrolysis. They can be separated and identified by paper chromatography. The procedure for quantitative estimation after paper chromatographic separation is suitable for quantities of 2—20 μg. This procedure has been applied to organophosphate standards added to lettuce, apples, and strawberries. Recoveries varied from 84 to 100% of the added compound.
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