Abstract

A combined gas-liquid chromatography and enzyme procedure is described for the determination of organophosphorus compounds that show anticarboxylesterase activity in vitro. The method is based on the enzyme hydrolysis of 1-naphthyl acetate and on the determination of both the released 1-naphthol and the unhydrolyzed 1-naphthyl acetate by gas-liquid chromatography. With bovine liver as the source of enzyme the detection limit for paraoxon was 13 ppb.

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