Determination of parameters of chick embryos used in the production of inactivated influenza vaccines

Abstract

The results of determining the parameters of chicken embryos for obtaining inactivated influenza vaccines are presented. The studies were carried out at LLC "Fort" (Ryazan region). 8.736 million chicken embryos were used from chickens of egg crosses Rhodonit-3, Hisex brown. In the course of the research, the dependence of the development of a chicken embryo on various growth periods was revealed. It has been established that the optimal parameters for the use of chicken embryos in the production of influenza vaccines are the life span of 7–9 days, the weight of the embryo is 2–3 g. high yield of hemagglutinin (up to 70%) compared to other groups of the sample (P<0.001). It has been shown that the increase in allantoic fluid largely depends on the purification systems used in production. The most optimal cleaning method is a tangential filtration system, which ensures high purity of the substrate obtained using chick embryos.