Abstract
Solid substrate room-temperature phosphorimetry on a poly(vinyl alcohol) (PVA) substrate was applied to the analysis of p-aminobenzoic acid (PABA) in the presence of bovine serum albumin (BSA). The buffer solution containing PABA and BSA was spotted on the PVA substrate and after sufficient drying, room-temperature phosphorescence (RTP) measurements were performed on both the front and the back side of the substrate. The BSA was separated on the front surface of the substrate and it emitted a negligibly weak RTP signal. Therefore, the RTP measurement of PABA on the reverse of the sample spotted side was not interfered by BSA, whereas, impurities in the BSA permeated into the PVA and emitted a weak RTP signal on the back side of the substrate. However, the RTP intensities of the impurities were weak enough as compared to that of PABA under the experimental conditions, so that their interference could be neglected. To improve the RTP intensity and reproducibility of PABA in the presence of BSA, a new substrate that consisted of Millipore-VS and PVA layer was prepared. The analytical figures of merit of this substrate obtained under the best experimental condition for maximum phosphorescence emission are a relative standard deviation of 6.0% ( n=5) at a level of 2 nmol/spot and a linear calibration curve from 0.5 to 20 nmol/spot with a correlation coefficient of 0.996 for 25 data points.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
