Determination of Organochlorine Pesticides in Saposhnikovia Divaricata by Ultrasonic-Assisted Extraction Coupled with GC-ECD

Abstract

A simple, rapid, and sensitive method for determination of organochlorine pesticides in Saposhnikovia divaricata by ultrasonic-assisted extraction coupled with GC-ECD was developed and validated. The extracting agent was a mixture solvent of acetone–methylene chloride (1 + 1). The extracts were cleaned up with concentrated sulfuric acid as sulfonating agent. The samples were separated by the capillary column HP-5. 6 organochlorine pesticides in Saposhnikovia divaricata were determined by gas chromatography with an electron capture detector (GC-ECD) simultaneously. 6 pesticides reference substances had good linear relationship in the concentration range of 8–150 ng/g. The average recoveries were, respectively, 83.5–97.3 %, 84.4–99.7 %, and 89.9–98.7 % at three added levels of 15, 30, and 60 ng/g. Relative standard deviation (RSD) was, respectively, 3.0–7.3 %, 4.6–7.4 %, and 3.9–8.7 %. The method was simple, rapid, and high selectivity. It was suitable for determination of organochlorine pesticides in Saposhnikovia divaricata.