Abstract
A sensitive and reproducible high performance liquid chromatography (HPLC) assay was developed for the determination of cell-associated levels of the new fluoroquinolone antimicrobial agent, OPC-17116 (Otsuka Pharmaceutical Co. Ltd.). The assay consisted of a reverse phase C18 analytical column with fluorescence detection at 288 nm and was used to measure levels of OPC-17116 in the constituents of bronchoalveolar lavage (epithelial lining fluid and alveolar macrophages). Thus it was possible to measure drug concentration at specific sites in the respiratory tree. This is especially important because of differences that may exist between these levels and those found in serum. In addition, a good correlation was found between the HPLC assay and an established microbiological assay.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
