Abstract
A validated, simple and highly sensitive analytical method was described for the determination of Ochratoxin A (OTA) in various wines produced in Turkey. OTA concentrations of 25 wine samples were examined using high performance liquid chromatography coupled with fluorescence detection. OTA and diflunisal (internal standard) were separated using isocratic elution mode with a reversed phase Nucleosil® C18 column. The mobile phase consisted of CH3CN : H2O : CH3COOH (50 : 48 : 2, v/v/v) was pumped at a flow rate of 0.4 mL/min. The analytes were detected at 330 nm excitation and 450 nm emission wavelengths within an average time of 13 min. Samples were prepared by simply filtrating the wine samples through a 0.2 μm filter and injected into the system without further extraction or concentration steps. OTA was detected in μg/L levels with adequate chromatographic resolution. It was found that the amount of OTA was higher than the permitted limits (< 2 μg/L) in 14 out of 25 samples, especially in red wines.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
