Abstract
A novel method for the determination of nucleic acid at nanogram levels was developed based on the measurement of resonance light scattering (RLS) signals of 3,3′-dichlorobenzidine (DCB). In the Britton-Robinson buffer (pH 2.21), the weak light scattering of DCB was greatly enhanced by addition of calf thymus DNA (ctDNA), the maximum RLS peak is at 346 nm and the enhanced intensity of RLS is in proportion to the concentration of ctDNA. The linear range is 0.05–5 µg mL−1 for ctDNA, and the detection limit is 14 ng mL−1 (3σ). DNA in synthetic samples was analyzed with satisfactory results.
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