Abstract
ABSTRACT A new quantitative determination method at nanogram levels for nucleic acids in aqueous solutions, based on the enhancement of Resonance Light-Scattering (RLS) of Night Blue (NB) by nucleic acids, has been established. The detection limits for calf thymus DNA and yeast RNA is as little as 1.8 and 2.2 ng ml−1, respectively. In addition to its high sensitivity, this method have other advantages: wide pH range (4.5–7.0), rapid reaction (within 10 s), stable mixtures formed (more than 2 days), simple operation (one-step assay), little interference from proteins, nucleosides and most metal ions. The maximum enhanced RLS signal is near 400 nm and 550 nm. The enhanced RLS is proportional to the concentration of nucleic acids in the range of 0–1.2 µg ml−1 for calf thymus (ctDNA) and 0–2.5 µg ml−1 for yeast RNA (yRNA). Four synthetic and real samples were determined with satisfaction results.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
