Abstract
A CO 2 equilibration curve was derived to examine in vitro and in vivo buffering characteristics of whole chicken blood at 40.0° C. Blood equilibrated with CO 2 in vitro exhibited linear relationships on both log P CO 2 /pH and HCO 3 −/pH graphs, with slopes of −1.45 and −20.2, respectively. When arterial blood samples were drawn from anaesthetized but spontaneously breathing animals, a plot of the log P CO 2 ; and pH of these samples was linear from 18 to 75 mm Hg had a slope of −1.24. When these same data were plotted as [HCO − 3] p/pH the scatter was so great that linearity was difficult to substantiate. Changes in hemoglobin saturation from 100% to 60% S O 2 produced no change in buffering ability of in vivo chicken blood. Determining acid-base status of the chicken by the log P CO 2 /pH graph is possible so long as the reference region along the buffer line is related to the state of the animal, e.g., conscious or anesthetized because increases of arterial blood P CO 2 vary with the degree of anesthetization.
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