Abstract
AbstractFurazolidone is a broad‐spectrum antibiotic that is frequently used in aquaculture on account of its excellent antibacterial properties. In this study, both the enzyme‐linked immunosorbent assay (ELISA) and high‐performance liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) methods were used to analyze the content of residual 3‐amino‐2‐oxazolidinone (AOZ), a metabolite of furazolidone in Tilapia tissue. Homogenized fish samples were spiked with various amounts of AOZ, and following combined acid‐hydrolysis and derivatization of the homogenized tissue with 2‐NBA (2‐nitrobenzaldehyde), sample clean‐up was performed and the derived 2‐nitrophenylmethylene‐3‐amino‐2‐oxazolidinone (NPAOZ) was analyzed. Using the LC‐MS/MS method, a linear correlation between measured concentration Y and spiked concentration × was observed: Y = 0.4518X − 0.0166, R2 = 0.9972. The linear equation for the ELISA method was Y = 0.9322X + 0.5168, R2 = 0.9066. These results demonstrated that the ELISA method might overestimate the residual AOZ content at low concentrations. The detection limit and recovery of the known addition were 0.05 μg kg−1a and 108% for the LC‐MS/MS method and 0.31 μg kg−1 and 305% for the ELISA method, respectively.
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