Abstract
An assay for determination of one of the styrene mercapturic acids [(N-acetyl-S-(2-phenyl-2-hydroxyethyl)cysteine] was developed. Styrene mercapturic acid in urine samples was determined using high performance liquid chromatography (HPLC) with electrochemical detection. Urine samples from 6 individuals who had been experimentally exposed to styrene (205–220 mg/m 3, 2 hrs, 50 W) were analysed using our assay. The absorption of styrene during the exposure was 3.6 mmol (range 3.2–4.1) or 64% of the amount inhaled. N-acetyl-S-(2-phenyl-2-hydroxyethyl)cysteine could not be identified in the urine collected during the exposure and up to 5 hrs from the end of the exposure. If present, the metabolic yield from absorbed styrene was less than 1%. Our results, in contrast to what have been reported from animal experiments, indicate that, in man, gluthatione conjugation of styrene to mercapturic acids is a metabolic pathway of less importance and/or that styrene-glutathione conjugates in man undergo a metabolism different from that which has been reported in rats.
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