Abstract
An analytical method based on solid-phase extraction–hydrophilic interaction chromatography–tandem mass spectrometry (SPE–HILIC–MS/MS) has been developed to quantify moniliformin in vegetable oil. The sample was extracted by methanol and purified by an Oasis MAX solid-phase extraction column. Following purification, it was separated by a Waters HILIC hydrophilic interaction column. Acetonitrile and 0.5 mM ammonium acetate solution were used as a mobile phase for gradient elution. An electrospray-negative ion multiple-reaction monitoring mode was used to quantify moniliformin by external standard method. In the mass concentration range of 0.1–10 μg/L, the correlation coefficient of moniliformin was 0.9997. The detection limit of this method was 0.03 μg/L, and the limit of quantification was 0.1 μg/L. When the three levels of 1.0, 2.0 and 5.0 μg/kg moniliformin were added, the recovery was 84.6%–98.2% with a relative standard deviation of 1.3%–5.1%. The results indicated that this method is simple, fast, sensitive, reproducible, and can be used to determine moniliformin in vegetable oil.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.