Determination of Minimum Effective Concentration of Cashew Nut Shell (CNS) Pyrolysis Products for Antibacterial Escherichia coli Using Kinetics Approach

Abstract

Determination of minimum effective concentration of cashew nut shell (CNS) pyrolysis products as an antibacterial Escherichia coli using kinetics approach has been done. The purpose of this study is to determine minimum concentration of CNS pyrolysis products which are effective as antibacterial E. coli using chemical kinetics and determine reaction order (n) and rate constant (k), equipped with the rate of reaction equation. And it also determine the relation of initial concentration [A]o, concentration in time [A]t and time variable (t). The results showed that the CNS pyrolysis products consist of two groups: phenolic compounds and alkane compounds. GCMS results also showed that main constituent of the compound is m-octyl-phenol (13.86%). Inhibitory zone on variation in concentration of 100%, 75%, 50%, 25% and 12.5% was 1.47; 1.20; 1.19; 0.87; and 0.75 cm respectively. Reaction order (n) = 0.3 and rate constants (k) = 3.3 so reaction rate equations is r = 3.3 [A]0.3. Relations of initial concentration [A]o and concentration in time [A]t and time variable (t) obtained [A]t = [A]o-0.05t. Minimum concentration making the CNS pyrolysis products effective as an antibacterial E. coli is 24.06%.