Abstract
A method is described for the simultaneous determination of seven metals - Al, Co, Cr, Mn, Mo, Ni, and V - in blood serum using a simultaneous, multielement atomic absorption spectrometer (SIMAAC) and graphite furnace atomization. Our original intention was to determine two additional metals - Pb and Sn - however, the high ashing temperature needed resulted in premature loss of these volatile metals. Serum samples (2 mL) are dry-ashed with 20 ..mu..L of Mg(NO/sub 3/)/sub 2/.6H/sub 2/O (20 g/L) as an ashing aid/matrix modifier. The organic-free residue is reconsituted with 0.5 mL of nitric acid (50 mL/L), allowing for a fourfold concentration step. The addition of Mg(NO/sub 3/)/sub 2/ permits a graphite furnace ashing temperature of 1300/sup 0/C. Atomization from a L'vov platform with a fast heating rate and the use of peak area measurements permit calibration with acidifed aqueous standards. A well-characterized bovine serum pool is used to establish the accuracy and precision of the method. Serum samples from 30 fasting adult volunteers, drawn under controlled conditions, are analyzed to assess the efficacy of this method to determine these seven metals over the ranges normally encountered in human sera. 16 references, 1 figure, 8 tables.
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