Abstract
Isocratic column liquid chromatography on poly(styrene-divinylbenzene) copolymer allowed complete separation of metacycline, 4-epimetacycline, oxytetracycline, doxycycline and 6-epidoxycycline. 2-Acetyl-2-decarboxamidometacycline was eluted on the tail of metacycline. The mobile phase was 2-methyl-2-propanol-0.2 M phosphate buffer (pH 9.0)-0.01 M sodium ethylenediaminetetraacetate (pH 9.0)-water (2.5:10:10:77.5, m/v/v/v). The flow-rate was 1.0 ml/min and detection was performed at 254 nm. Official standards were compared and a number of commercial bulk samples and specialties were analysed. 2-Acetyl-2-decarboxamidometacycline, 6-epidoxy-cycline and doxycycline were the main impurities, while 4-epimetacycline and oxytetracycline were minor impurities.
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