Abstract
Cryptococcus neoformans is best known as a life threatening pathogen that infest majorly immuocompromised individual. In this work, two isolates; one clinical CD4832 and environmental NMB5 selected and confirmed based on their melanin production on niger seed agar and ability to grow at 37°C. These selected isolates were involved in antifungal susceptibility test using Fluconazole as the antibiotic agent. Response to Fluconazole showed that the CD4832 is less susceptible compare to NMB5. The results clearly demonstrated that the two strains have different susceptibility levels based on the various concentration of the Fluconazole that was used. In attempting to check if the two strains have the ability to recombine, they were mixed together and treated with the same concentration of Fluconazole. Interestingly, the results suggested two mating types- thus opening wide discussion for research into new drug target.Keywords: Mating types, virulence, antifungal agent, sexual recombination
Highlights
Cryptococcus neoformans are the main causative agents of Cryptocococcosis
Mating types and serotypes have both been implicated as virulence factors in C. neoformans (Xlin et al, 2008)
Since antifungal susceptibility tests has been used in identifying serotypes, here in this work, attempt has been made to identifying mating types, using the reaction of strain to fluconazole
Summary
Cryptococcus neoformans are the main causative agents of Cryptocococcosis. Cryptococcus is a basidiomycetes yeast that causes infections after inhalation of its basidiospores found in the environment (Martinez and Casidevall, 2006; Giles et al, 2009; Negroni, 2012). Keywords; Mating types, virulence, antifungal agent, sexual recombination Differences in biology, virulence, clinical features, epidiomology, drug susceptibilities have been reported to be related to mating types varieties species and molecular types within the Cryptococcus species complex (Kwon-Chung et al, 1992; Speed and Dunt, 1995; Casadevall and Perfect, 1998; Meyer et al, 2003; Tritles et al, 2004; Campbell et al, 2005; Fraser et al, 2005).
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