Abstract

Heliothis moths raised on nutrient media containing various concentrations of rubidium chloride were individually digested with nitric acid and rubidium was determined by either atomic-absorption spectrometry (AAS) with a carbon rod atomiser or inductively coupled plasma atomic-emission spectrometry (ICP-AES). Conditions were established such that interference-free determinations were possible by both methods. Recovery estimates were not significantly different from 100%. Instrumental precisions for single sample assays were acceptable for use in entomological marking experiments. Moths were best analysed initially by ICP-AES. Those moths containing less than 15 µg of rubidium were then analysed by AAS.

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