Abstract
In this study, manganese was extracted in the blood of rats based on desferrioxamine (DFO), deferasirox (DFX) and deferiprone (DFP) as chelators (ligands) by ionic liquid-liquid phase extraction method (ILLEM) before being determined by F-AAS. Also, the toxic effects of manganese on blood serum and hematology parameters such as RBC, WBC, HGB, PLT and HCT were investigated. Male Wistar rats were randomly divided into control and toxic groups. Manganese chloride was administrated orally in low and high doses. Orally (deferasirox and deferiprone) or intraperitoneally (desferrioxamine) for 2 weeks. Results showed that exposure to manganese significantly increased both counts of hematology parameters and concentration of this metal ion in serum compared to the control group. First, manganese was chelated with ligands and then the hydrophobic ionic liquid (IL, [HMIM][PF6]) was added to rat blood samples, after shaking and centrifuging, the upper liquid phase was separated by auto-sampler and mixture of IL/ligand in the bottom of conical tube back-extracted and determined with F-AAS. The linear range, LOD and enrichment factor for 10 mL of blood rats were obtained 25- 180 μg L-1, 6.5 μg L-1 and 19.92, respectively. By chelation therapy manganese was removed from serum and hematology parameters were normal level.
Highlights
Trace elements like manganese are essential for normal development and body function across the life span, and are widely distributed in the tissues
Manganese leaving the enterocyte and entering the circulation bound to transferrin, may bind to transferrin receptors on erythroid cells
We studied the effect of MnCl2 exposure in low and high doses on blood serum and hematology parameters in male Wistar rats
Summary
Trace elements like manganese are essential for normal development and body function across the life span, and are widely distributed in the tissues. Chelation therapy that could be carried out as a single and/or combined therapy involves the use of chelating drugs that bind metal for the treatment of potentially fatal conditions [10,11] In this investigation, we studied the effect of MnCl2 exposure in low and high doses on blood serum and hematology parameters in male Wistar rats. Due to the trace concentration of manganese, and difficulty matrixes, the sample treatment used before sample analysis Many sample treatments such as, the liquid-liquid extraction [21], the deep eutectic solvent extraction [22], the column graphene oxide-based solid phase extraction [23] were used for manganese extraction in blood samples. The toxic effects of manganese on blood serum and hematology parameters such as: RBC, WBC, HGB, PLT and HCT were investigated
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