Abstract
A method for the determination of maleic hydrazide(MH)in tobacco and tobacco products was established,in which MH in tobacco sample was extracted with a solvent,and cleaned up by a solid phase extraction cartridge,then detected with high performance liquid chromatography.The residue of MH in 23 tobacco samples was determined.The results showed that: 1)Good results were obtainable when tobacco sample was extracted with 4 mol/L hydrochloric acid under heating and refluxing for 1 hour.2)The MH peak was completely separated from impurity peaks in 20 minutes by reversed-phase HPLC with a Varian Microsorb-MV 100-5 C18 column under isocratic elution with 0.1 mol/L acetic acid as mobile phase at a flow-rate of 0.7 mL/min.3)The recovery of MH was 97.11% with the relative standard deviation(RSD)of 7.13%.This method is suitable for the determination of MH in tobacco and tobacco products.
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