Abstract
A fluorescence high performance liquid chromatographic (HPLC) method was developed to separate and quantify maleic hydrazide (MH) in potatoes and onions. Methanol was used as the extraction solvent. A 3-mL aliquot of the extract was dried under nitrogen and the residue was reconstituted in 3-mL of water by sonication. The water fraction was passed through a C18 solid phase extraction (SPE) tube to remove interfering compounds. Onions and potatoes fortified from 2.5 to 20 ppm showed percent recoveries ranging from 66 to 94. Within-day and between-day reproducibility studies run at 2.5, 5.0, 10.0, 15.0, and 20.0 ppm indicated the procedure was reproducible yielding percent coefficients of variation (%CV's) ranging from 1.3 to 10. The limit of detection was 0.5 ppm. A comparison was made between this HPLC method and a capillary electrophoresis (CE) method. The linear regression for MH was y = 0.816x + 1.071 with a correlation coefficient of 0.878.
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