Abstract
ABSTRACTAn adsorptive stripping voltammetric method for the determination of malachite green in aquaculture water has been developed. Initial studies were made using the cyclic voltammetry of malachite green at a glassy carbon electrode in 0.1 M phosphate buffer from pH 2 to 10. The redox behavior observed for malachite green was verified by the characterization of malachite green and its reduction product, leucomalachite green. Furthermore, leucomalachite green was found not to interfere with the determination of malachite green at pH 7.4, the optimum pH for malachite green determination. As a result, further studies were performed using adsorptive stripping voltammetry for the determination of malachite green in aquaculture water. The voltammetric waveform, accumulation potential, and accumulation time were optimized. The calibration plot was linear from 0.2 µM to 1.2 µM for malachite green using differential pulse voltammetry with a sensitivity of 0.8311 µA/µM. Using the method of multiple standard addition, aquaculture water fortified with 0.5 µM and 0.75 µM malachite green provided mean recoveries of 78.79% and 87.20% with coefficients of variation of 2.07% and 1.45%. Therefore, analytical figures of merit suggest that this method provides rapid, simple, economical, and precise determination of malachite green in aquaculture water.
Highlights
Malachite green (C23H25N2Cl) is a member of the triphenylmethane class of dyes and is known to exhibit both antimicrobial and antiparasitic properties
There was very little response for leucomalachite green seen by cyclic voltammetry at pH 7.4, the pH at which we undertook the investigation of malachite green
Leucomalachite green would not interfere with the determination of malachite green
Summary
Malachite green (C23H25N2Cl) is a member of the triphenylmethane class of dyes and is known to exhibit both antimicrobial and antiparasitic properties. It has been extensively used as a biocide in the aquaculture industry world-wide (Aiderman 1985). In addition to its use in aquaculture, malachite green has been widely used as a dye in the textile industry, as well as a food additive and colouring agent (Culp and Beland 1996). In order to preserve the environment and protect human health, it is very important to develop analytical methods for malachite green analysis
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