Abstract
The highly overlapping chromatograms and spectra of the major metabolites of asprin, salicylic acid (SA) and gentisic acid (GA) in the presence of uncalibrated and interfering pyrocatechuic acid (PA) were resolved using HPLC-DAD combined with SWATLD algorithm. The elution time was set from 0.6628 min to 0.9495 min and the detection wavelength was chosen from 268 nm to 332 nm. Both SA and GA were determined simultaneously in aqueous solution with their recoveries were (100.5 ± 6.7) % and (109.2 ± 5.3) %, respectively. The satisfying results indicate this method can be easily performed and applied to solving second-order calibration problems quickly and accurately.
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