Abstract

The stability constants for the binding of magnesium ions to phospho enolpyruvate, 2- and 3-phosphoglycerates, 2,3-diphosphoglycerate, P i, glucose 6-phosphate, isocitrate, glutamate and NAD have been determined spectrophotometrically in the presence of 8-hydroxyquinoline at 25 and 37°C. Estimates of potassium binding constants have also been calculated for phospho enolpyruvate and 2- and 3-phosphoglycerate and compared with previously published values.

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