Determination of Lymphocyte Cytokinesis-Block Micronucleus Values in Apparently Healthy Children by means of Age and Sex.

Burak Durmaz,Ozgur Cogulu,Mehmet Orman,Guldane Koturoglu,Cumhur Gunduz,Hasan Taslidere

doi:10.1155/2019/8729561

Abstract

The cytokinesis-block micronucleus (MN) assay on blood lymphocytes is one of the most important tests implemented in cytogenetics for the measurement of genotoxicity. For the purpose of biological dosing, it is crucial to know the spontaneous frequency of MN and its normal values in general population, especially in children, which are used for the population databases. In this study, MN levels were investigated in cytokinesis-blocked lymphocytes of 150 apparently healthy children aged 1 to 15. Our aim was to assess the variability of MN values according to age and sex. The mean MN frequency among boys was 3.69 ± 1.747‰ and 4.12 ± 1.867‰ in girls where there was no significant difference in relation to age and sex. However, when we separated age groups as 0–2 years, 3–5 years, 6–10 years, and 11–15 years, one-way ANOVA test showed significant association. Significance was obvious in the 0–2 years age group with the 3–5 years age group and 6–10 years age group. When we grouped our study population as 0–2 years and 3–15 years, the mean MN frequency among the 0–2 years age group was 2.85 ± 1.599‰ and 4.07 ± 1.867‰ in the 3–15 years age group which was also statistically significant. This difference may be attributed to age-related increase of close contact with environmental hazardous agents. In conclusion, normal values of MN obtained in this study will add valuable information in regard to update the current childhood population data and will act as a reference for further genotoxicity studies.

Highlights

Maintaining the normal function of a cell is crucial for a living organism, and it depends largely on proper DNA replication and repair mechanisms
We are increasingly exposed to genotoxic effects including ionizing radiation, detrimental chemicals, and harmful physical agents. ese genotoxic agents gradually damage the genetic information within a cell and may predispose to cancer, chronic diseases, or apoptosis [1, 2]. e effect of those factors on genomic stability could be determined by the measurement of micronuclei (MN) [3]
Elevated MN frequency in lymphocytes has been shown to be associated with an increased risk of cancer, severe adverse cardiovascular events in coronary artery disease patients, diabetes, mild cognitive impairment, Alzheimer’s, and Parkinson’s diseases [5,6,7,8]

Summary

Research Article

Burak Durmaz ,1 Hasan Taslidere, Guldane Koturoglu, Cumhur Gunduz, Mehmet Orman, and Ozgur Cogulu 1,2. E cytokinesis-block micronucleus (MN) assay on blood lymphocytes is one of the most important tests implemented in cytogenetics for the measurement of genotoxicity. MN levels were investigated in cytokinesis-blocked lymphocytes of 150 apparently healthy children aged 1 to 15. E mean MN frequency among boys was 3.69 ± 1.747‰ and 4.12 ± 1.867‰ in girls where there was no significant difference in relation to age and sex. When we grouped our study population as 0–2 years and 3–15 years, the mean MN frequency among the 0–2 years age group was 2.85 ± 1.599‰ and 4.07 ± 1.867‰ in the 3–15 years age group which was statistically significant. Normal values of MN obtained in this study will add valuable information in regard to update the current childhood population data and will act as a reference for further genotoxicity studies

Introduction

Materials and Methods

Male Female Total