Abstract
Amaryllidaceae is one of the most widely used medicinal plant family [1]. Sternbergia candida Mathew & T. Baytop (Sc) is an endemic member of the family from Mugla, Turkey [2]. Lycorine is the major alkaloid of Sternbergia species. HPLC analysis of the bulbs of the species has been done previously [3]. In this study, it is aimed to determine the lycorine in chloroform and methanolic extracts of both bulbs (SCBC: SCBM) and leaves (SCLM) of Sc by HPLC. The analysis was performed with a shorter column (3µm, C18 150mm x 3mm) in a gradient solvent system without acetonitrile (A: 97.5% 10 mM ammonium bicarbonate with 2.5% methanol; B: 2.5% 10 mM ammonium bicarbonate with 97.5% methanol pH: 7.8) with a flow rate of 0.3ml/min. The results are given in Table 1. Lycorine was found in the leaves of the plant however it was higher in the methanolic extract of the bulbs.
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