Abstract
It is showed that in the buffer solution of Borax-HCl (pH=6.00), the presence of LU could quench the fluorescence of OFLO, and the quenched level is linear with the concentration of luteolin in a certain range. The detection limit of luteolin is 1.27×10-9g·mL-1.The linear range of luteolin is 2.00×10-7- 8.00×10-5mol·L-1with R =0.9955. The average recovery is 99.2%, RSD = 0.75% . The average content of luteolin in honeysuckle is 212.0μg·g-1. At the same time, its mechanism is discussed by resonance light scattering and absorption spectra. Compared with other analysis methods for Luteolin which have been reported,this method has a relatively wider linear range and higher sensitivity.
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