Abstract
Objective: The objective of this research was to investigate the extraction and isolation of chemical compounds of Zanthoxylum limonella Alstonextracts which selected from Chiang Mai and Pa Yao province, Thailand and Taiwan.Method: There are 3 locations of medicinal plant, Z. limonella Alston was collected, two provinces of northern Thailand, Chiang Mai and Phayao,including the other country, Taiwan. All of extractions were carried out by methanol. In this experimental study, thin-layer chromatography (TLC) andhigh-performance liquid chromatography (HPLC) were measured.Results: The result was found that the best organic solvent for extraction is methanol. The suitable of extraction was methanol and toluene in the ratioof 9:1 when developed with TLC and gradient methanol and water when analyzed with HPLC. The potential bioactive compound from crude extractedwas lupeol. Crude extracted from leaf at Chiang Mai was highest lupeol as 5655.72 μg/mg extraction whereas crude extracted from leaf at Phayaoshowed this compound as 16, 98.81 μg/mg extraction. In addition, the crude extraction from root and bark of both areas showed the amount of lupeolless than leaf crude extracted. The further study will focus on antimicrobial activity and biological activity in the near future.Conclusion: The bioactive compound, lupeol was found in all part of Makhaen in Thailand but did not found in Taiwan seed.
Highlights
Zanthoxylum limonella Alston (Rutaceae) is medical plants, located in tropical and subtropical areas
The suitable of extraction was methanol and toluene in the ratio of 9:1 when developed with thin-layer chromatography (TLC) and gradient methanol and water when analyzed with high-performance liquid chromatography (HPLC)
Crude extracted from leaf at Chiang Mai was highest lupeol as 5655.72 μg/mg extraction whereas crude extracted from leaf at Phayao showed this compound as 16, 98.81 μg/mg extraction
Summary
Zanthoxylum limonella Alston (Rutaceae) is medical plants, located in tropical and subtropical areas. In the past, this plant has been extensively used as a folk medicine for different medical purposes such as stomach ache, toothache, intestinal worms, rheumatism, scabies, snakebites, fever, and cholera [1]. In Thailand, Z. limonella Alston, locally called makhwaen, is widely distributed in the northern part of Thailand and has been traditionally used in food as spices. The reports on pharmacological properties and functional characteristic of Z. limonella have not been whole preformed in Thailand and South East Asia. We present data on the bioactive compounds of the crude extracts of Z. limonella Aston in Thailand compared with Chinese plant species at Taiwan
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