Abstract
An appropriate procedure was developed to evaluate low molecular weight organic acids (LMWOA) in soils. The proposed method includes water extraction, purification, and determination. To minimize any chemical modification during extraction, water extraction was carried out at a soilwater ratio of 1:5 for 1 h of reciprocal shaking at room temperature. Purification was carried out using ion exchange and octadecylsilane (ODS) columns. Determination of LMWOA was conducted by HPLC at UV 210 nm and an anion exchange column was used for oxalic acid, while a ODS column was used for the other seven organic acids (citric, succinic, maleic, lactic, malonic, fumaric, and malic acids). The amount and composition of the LMWOA were generally similar between air-dried and freeze-dried soils, while fresh soil gave a smaller amount and a different composition of LMWOA.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
