Abstract
Using a calcium-sensitive electrode, the determination of ionic calcium in human serum was investigated. Serum samples obtained from 17 healthy individuals yielded the following data: total calcium 9.63 ± 1.11 mg 100 ml ; ionized calcium 4.72 ± 0.61 mg 100 ml ; and bound calcium, 4.91 ± 0.83 mg 100 ml . The proportion of ionized and bound calcium as a percentage of total calcium was 49.24 ± 5.08% and 50.76 ± 5.02%, respectively. It was concluded that the electrode method of measuring ionized calcium is probably the simplest and most practicable.
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