Abstract
Calcium homeostasis in brain is altered in many conditions, but there is no method to assay quantitatively local calcium flux into brain in vivo. 45Ca uptake into gross-dissected brain regions was measured and compared with results obtained with a quantitative autoradiographic procedure developed to assay influx of 45Ca into brain. Regional calcium contents, brain-to-plasma distribution ratios for calcium and 45Ca, apparent plasma-to-brain transfer coefficients, and net uptake of 45Ca into gross-dissected regions varied by as much as 80%. Local rates of net uptake of 45Ca into 34 structures determined by autoradiography varied by 12- to 14-fold, and rates of movement of 45Ca down concentrations gradients varied by a factor of 7. Previous studies with gross-dissected brain regions suggested changes in calcium uptake into brain during aging, but the values of all the variables assayed in the present study were similar in young adult, middle-aged, and old male rats. A quantitative autoradiographic procedure to assay levels of 45Ca in brain provides the anatomical resolution required to investigate local calcium flux in a variety of physiological and pathological conditions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.