Abstract
A sensitive and eco-friendly method was developed for the spectrophotometric determination of Lisinopril (LSP) in bulk and pharmaceutical formulations by cloud point extraction technique. The method was based on the formation of a blue-colored coordination complex between Lisinopril (LSP) and Cobalt Thiocyanate (CTC) at a suitable pH. The Complex in aqueous medium was extracted into surfactant layer by cloud point extraction using a non-ionic surfactant Triton X-114 and then the surfactant layer was dissolved in a suitable volume of ethanol and the amount of Lisinopril was determined spectrophotometrically at a wavelength of 625 nm. The conditions like concentration of the drug, concentration of CTC and of Triton X-114, PH, etc. were optimized by OFAT (One Factor At a Time) method. The linear range of calibration curve was 1 - 6 μg/ml and the linear regression equation with a correlation coefficient of 0.99996 was y = 0.0021 + 0.084x. Preconcentration and enrichment factors were found to be 100 and 3.12 respectively, achieving the detection limit of 0.0588 μg/ml. The proposed method was successfully applied for the determination of LSP in the drug formulations. The obtained values were in agreement with the values as quoted by the manufacturers.
Highlights
For the body’s production of angiotensin II, Angiotensin converting enzyme inhibitors (ACE inhibitors) drugs are applied
The method was based on the formation of coordination complex between LSP and Cobalt Thiocyanate (CTC) at a suitable pH followed the extraction of the complex by cloud point extraction using Triton X-114 under the optimum conditions
The results showed that the absorbance of complex in the surfactant layer increased as shown in Figure 3 with increasing CTC concentration with subsequent increase of absorbance in the aqueous layer
Summary
For the body’s production of angiotensin II, Angiotensin converting enzyme inhibitors (ACE inhibitors) drugs are applied. Lisinopril (LSP) is a drug of angiotensin converting enzyme inhibitors class used primarily in the treatment of high blood pressure and heart failures and after heart attacks. Low-cost and green method was developed for the spectrophotometric determination of Lisinopril using CTC by cloud point extraction technique. There are a few reports available for the determination of drugs by CPE coupled with spectrophotometry [24] [25], spectrofluorometry and RP-HPLC [26] [27] [28] [29] [30]. A new, sensitive and green method was developed for the determination of Lisinopril in bulk and pharmaceutical formulations by CPE in combination with UV-Vis spectrophotometry. The proposed method was applied for the determination of LSP in pharmaceutical formulations
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