Determination of liquiritigenin by ultra high performance liquid chromatography coupled with triple quadrupole mass spectrometry: Application to a linear pharmacokinetic study of liquiritigenin in rat plasma.

Abstract

A simple, sensitive and rapid ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method has been developed and validated for the quantification of liquiritigenin, a promising anti-tumor agent. Liquiritigenin and the internal standard were separated on an Agilent Extend C18 column and eluted with a gradient mobile phase system of acetonitrile and water. The analysis was performed on a negative ionization electrospray mass spectrometer via multiple reaction monitoring (MRM). Transitions of m/z 255.0→119.0 for liquiritigenin and m/z 269.0→117.0 for the IS were monitored. One-step protein precipitation with acetonitrile was used to remove impurities and extract the analytes from plasma. The method had a chromatographic run time of 4.5min and a good linearity in the range of 1-1000ng/mL. The precision (R.S.D.) of intra-day and inter-day ranged from 4.54 to 10.65% and 5.94 to 13.81%, respectively; while the accuracy of intra-day and inter-day ranged from 104.06 to 109.28% and 94.98 to 112.05%. The recovery and stability were also within the acceptable limits. The validated method was applied to a linear pharmacokinetic study of liquiritigenin in rat plasma for the first time.