Abstract
It was reported in a previous paper that the peroxide value (POV) of a small amount of lipid can be determined by potentiometry in the JOCS tentative method for POV. The previous method was further modified in the present work to reduce the required amount of saturated potassium iodide aq. solution used for iodometry. Consequently, the time for titration was less, and the end point of titraion clearer, thus giving more reliable results.By the present method, it was possible to determine accurately the POV of a lipid extract from ca. 3 ml of human serum.Moreover, by the thiobarbituric acid (TBA) test for measuring peroxides indirectly, detection of peroxides in serum was possible at levels of less than 20% of the value by the present method. The present method makes possible the direct determination of a small amount of hydro-peroxide in serum lipid and thus makes it easier to understand the influence of lipid peroxidesin vivo on cancer, strokes, atheriosclerosis, the aging process etc.
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