Determination of levofloxacin in chicken tissue, manure and serum by self-ordered ring fluorescent microscopic imaging

Abstract

Based on the self-ordered ring (SOR) fluorescent microscopic imaging technique on a hydrophobic glass slide with Mn2+ and cetyltrimethyl ammonium bromide (CTMAB) as sensitizer, poly(vinyl alcohol)-124 (PVA-124) and NH3-NH4Cl buffer (pH 9.30) as the medium, a method has been developed for determining levofloxacin (LVFX) residues in chicken tissue, chicken manure and the concentrations in chicken serum. Sample preparation used the PBS and acetic acid-methanol as extracting agents for chicken breast muscle, liver and manure, respectively, the protein from chicken serum samples was removed with methanol. When the droplet volume is 0.2 μL, LVFX in the range of 5.7 × 10−14−1.0 × 10−13 mol/ring can be detected, and the limit of detection (LOD) can reach 5.7 × 10−15 mol/ring (2.8 × 10−8 M). Compared to reference [16], the sensitivity of SOR method enhances 1.7 times. It is a viable method for the determination of LVFX in chiken samples with the recoveries of 90.0–104.1% and relative standard deviations (RSDs) less than 3.6%. The results indicate that the method applied to chicken tissue, manure and serum is reliable and applicable.