Determination of lecithin: Cholesterol acyltransfer in plasma of two lizards: Tropidurus torqu atos (Iguanidae) and Ameiva ameiva (Teeidae)

Abstract

1. 1. The lecithin: cholesterol acyltransferase (LCAT) reaction is active in the plasma of both Tropidurus troquatos and Ameiva ameiva. 2. 2. LCAT in T. torquatos plasma was completely inhibited by 1 mM concentrations of disulphide, whereas the A. ameiva enzyme was only partially inhibited by disulphide at 10 mM concentrations. 3. 3. LCAT activity in T. torquatos plasma was measured by the Stokke- Norum procedure, whereas this method is counterindicated for A. ameiva LCAT, which instead was measured by the Glomset and Wright method. 4. 4. In both species absolute activity was variable but was of a similar order to that of LCAT in human plasma.