Abstract
A simple, selective and sensitive method was developed based on electrothermal atomic absorption spectrometry using Zeeman correction for quantitation of lead, in 100 ml of whole blood sample, as biological indicator for occupational exposure. Confidence parameters and stability of samples were considered. Ashing and atomization temperatures, considered critical, were 700 °C and 1,700 °C, respectively. The levels found during the validation process showed good sensitivity linearity, recovery, precision and accuracy. The stability results presented levels remaining constant for a 15 months period. The variations were not higher than 15% when comparing concentrations in zero time to those obtained after storage period.
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