Abstract
An LC–MS method was developed for routine determination of lactose in low-lactose and lactose-free dairy products. The lactose was separated on an amide column under an isocratic elution condition. Baseline separation of lactose from common interferents, including epilactose, lactulose, and allolactose were achieved under this LC conditions. The lactose chloride-35 adduct ions were detected by a single quadrupole MS in ESI- mode. 13C labeled lactose was used as an internal standard. The LOQ was estimated at 2.6 mg/100 g in a soy based infant formula. The recovery was found within the range of 90%–109% in the spiking study on commercial dairy products at spiking levels between 3.4 and 461 mg/100 g. The analysis of lactose concentration in the NIST SRM 1849a showed an accuracy of 109%. The RSD in the analysis of those commercial products were less than 4.3%. This method is sensitive and selective enough and fit for the routine determination of lactose in low-lactose and lactose-free dairy products.
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