Abstract
In order to establish a method to quantify labdanoids in oriental tobacco, a dispersive Solid-Phase Extraction-Gas Chromatography-Mass Spectrometry (d-SPE-GC/MS) method was used to measure ten labdanoids in oriental tobacco. The fragmentation mechanisms of nine labdaniols were deduced according to the fragmentation pattern of sclareolide, and their contents were semi-quantified by using the calibration curve of sclareolide. With anthracene-d10 as the internal standard, the effects of extraction time, eluent volume and purification on the contents of labdanoids were investigated. The results showed the correlation coefficient (R2) and relative standard deviation (RSD) of sclareolide was 0.999 5 and 2.09%, respectively. The recoveries ranged from 100.65% to 106.91%. The limits of detection and quantification were 0.06 μg·mL-1 and 0.19 μg·mL-1, respectively. This method is simple, sensitive, accurate, therefore suitable for the determination of labdanoids in oriental tobacco.
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