Determination of l-thyroxine in pharmaceutical preparations by flow injection analysis with chemiluminescence detection based on the enhancement of the luminol–KMnO4 reaction in a micellar medium

Abstract

A novel flow injection chemiluminescence (CL) method for the determination of l-thyroxine in the presence of cethyltrimethylammonium bromide (CTMAB) surfactant micelles is developed. The method is based on the significant signal enhancement of l-thyroxine on the luminol–KMnO4 system in alkaline solution sensitized by CTMAB. Parameters affecting the reproducibility and CL detection were optimized systematically. Under the optimum conditions, the net CL intensity versus l-thyroxine concentration was linear in the range of 5.0×10−8–3.0×10−6mol/L with the detection limit of 8.9×10−9mol/L. The sample throughput is calculated to be 140samples/h and the relative standard deviations (RSDs) for 13 replicate determination of 1.0×10−6l-thyroxine is 1.1%. The proposed method was successfully applied for the determination of l-thyroxine in pharmaceutical preparations with satisfactory recoveries in the range of 93.9–105.2%. This rapid, sensitive, and high throughput method would provide a new tool for l-thyroxine analysis.