Abstract
A kanamycin molecular imprinted SPR sensor was prepared with 4-vinylbenzeneboronic acid as the functional monomer, polyethylene glycol acrylate and 2,2′-azo isobutyl amidine hydrochloride as the crosslinker and the initiator, respectively. Underoptimumconditions, the responseperformance of the imprinted SPR sensor to kanamycin was evaluated. The imprinted sensor had a better imprinting effect and selectivity for kanamycin thanthe non-imprinted one. The linear range of imprinted sensor for kanamycin was from 1.0 × 10−7 to 1.0 × 10−5 mol/L (R2 = 0.9941). Based on a signal-to-noise ratio of 3, the detection limits of kanamycin were 4.33 × 10−8 mol/L for milk powder and 1.20 × 10−8 mol/L for honey, with the RSD as 1.18% and 0.31%, respectively.
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