Abstract
A reversed-phase column liquid chromatographic method was developed for analysis of isosorbide dinitrate (ISDN), isosorbide 5-mononitrate (IS5MN) and isosorbide 2-mononitrate (IS2MN) and its pharmaceuticals. Chromatography is performed to Bondclone ODS column using a mobile phase consisting of acetonitrile-PIC B6 buffer, pH 4.75 (13:87) with ultraviolet wavelength at 220 nm. The linearity calibration range was 0.05 mg/ml to 2.5 mg/ml (r = 0.9999), and recoveries were generally greater than 98.0%
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