Abstract
Abstract A reversed-phase high performance liquid chromatography (RP-HPLC) method was established for the determination of isoflavones (daidzein, glycitein, equol, and genistein) in human urine. After the addition of flavone as internal standard and the enzymatic hydrolysis in urine sample, the isoflavones were extracted with ethyl acetate (1:1) in medium of pH 7.0 and then separated by gradient elution with a ternary eluant of 0.02% trifluoroacetic acid-methanol-acetonitrile. The detection limits were 12.9 nM, 13.9 nM, 71.6 nM, and 11.8 nM for daidzein, glycitein, equol, and genistein, respectively. The recovery of the isoflavones was more than 89%. The proposed method is easier to carry out, accurate, reproducible, and suitable for the analysis of a large number of samples.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
