Abstract
A method is proposed for the determination of iron in biological material which makes use of the reaction between iron and thiogly-collic acid in an alkaline solution with the production of a purple color.Method for blood. Measure 0.2 cc. of blood into a pipette accurately calibrated to contain 0.2 cc. and deliver it into a Pyrex test tube, 20×180 mm., calibrated at 10, 15 and 20 cc. Add 1 cc. of 10 N sulfuric acid and introduce a small piece of silica or a glass bead to prevent bumping. Heat, preferably over a hot plate to evaporate the water. As soon as the material chars, allow to cool for 45 seconds and add 4 drops of 30% hydrogen peroxide drop by drop. Continue the heating for 5 minutes after the solution becomes colorless. Allow it to cool and add 10 cc. water. Into a similarly calibrated Pyrex test tube accurately measure 1 cc. of standard iron solution containing 0.1 mg. iron and dilute with distilled water to about the 15 cc. mark. Add one drop (0.05 cc.) of thioglycollic acid to both standard an...
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