Determination of Iron(II), Iron(III) and Total Iron in Some β-Thalassemia Patients Using Different Analytical Techniques

Abstract

There are many well-known analytical methods for determination of iron (II) and iron (III). A mong these methods: gravimetric, titrimetric, potentiometric, conductometric, batch and flow – injection spectrophotometric methods. In the present study, two batch spectrophotometric, atomic absorption spectrometric and biolabo kit methods have been used for determination of iron (II), iron (III) and total iron. The present methods have the advantages of high sensitivity, low cost reagent, low operation cost, simplicity, speed and their applications for determination of iron (II) and iron (III) in some serum samples of normal human and β-thallasemia patients in Erbil city. For the first time especially in Erbil city attempts were made to use zero, first and second derivative spectra to identify the serum samples of some β-thallasemia patients from the normal human serum samples due to the appearance and resolution of peaks in both cases.